CURATIVE EFFECT OF KAEMPFEROL-5-O-?-DGLUCOPYRANOSIDE FROM METHANOLIC EXTRACT OF PHYSALIS MINIMA LINN AGAINST ADJUVANT INDUCED ARTHRITIS IN ALBINO RATS
Shanmuga Sundaram M*, Dr. Karthi J, Dr. Swarnalatha S
Department of Pharmacology, Pallavan Pharmacy College, Iyyengarkulam, Kanchipuram – 631502
To investigate the in vitro anti-arthritic potential of isolated compound Kaempferol-5-O-?-Dglucopyranoside (K-5-?-D-g) from methanolic extract of aerial parts of Physalis Minima using Wistar rats. Methods: Anti-arthritic activity of K-5-?-D-g was evaluated using Freund’s complete adjuvant model. The parameters such as foot pad thickness, body weight measurement, rheumatoid factor, spleen index assessment, hematological estimation and histopathology of synovial joints were examined. Methotrexate was used as standard (5mg/kg b.w.p.o). Results: Administration of K-5-?-D-g at the doses of 10, 20, 50, 100 mg/kg b. w. p. o exhibited statistically significant (p < 0.001) inhibition of the paw edema, increase in body weight, decrease in arthritic, rheumatoid and spleen index factors. The results of haematological parameters showed that the treatment of K-5-?-D-g at varied doses significantly increased (P < 0.001) the Heamoglobin (Hb) level, Red Blood Cells (RBCs) count, decreased White Blood Cells (WBCs) count and Erythrocyte Sedimentation Rate (ESR). All these treatment groups were compared with arthritis treated groups. Histopathological analysis demonstrated that K-5-?-D-g had comparable anti-arthritic activity with methotrexate. Conclusion: The results obtained suggested that K-5-?-D-g from the methanol extract of Physalis minima holds significant anti-arthritic activity by inhibiting various inflammatory mediators and there by act on multiple targets to achieve optimal effects.
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