EVALUATION OF ANTI-OXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF WITHANIA SOMNIFERA WATER EXTRACT
Shauroseni Palchoudhuri
Department of Microbiology, Herbicure Healthcare BioHerbal Research Foundation, Boral, Garia, Kolkata-700154, West Bengal, India
Deesha Roy
Department of Molecular Biology, Herbicure Healthcare BioHerbal Research Foundation, Boral, Garia, Kolkata-700154, West Bengal, India
Kazi Atikur Rahman
Department of Molecular Biology, Herbicure Healthcare BioHerbal Research Foundation, Boral, Garia, Kolkata-700154, West Bengal, India
Debalina Sinha Roy
Department of Microbiology, Herbicure Healthcare BioHerbal Research Foundation, Boral, Garia, Kolkata-700154, West Bengal, India
Pinakpani Dasgupta
Department of Chemistry, Herbicure Healthcare BioHerbal Research Foundation, Boral, Garia, Kolkata-700154, West Bengal, India
Sukhen Das
Department of Physics, Jadavpur University, Kolkata 700032, India
Sujata Ghosh Dastidar
Department of Microbiology, Herbicure Healthcare BioHerbal Research Foundation, Boral, Garia, Kolkata-700154, West Bengal, India
Debanjan Mukhopadhyay
Department of Molecular Biology, Herbicure Healthcare BioHerbal Research Foundation, Boral, Garia, Kolkata-700154, West Bengal, India
This study aimed to evaluate the active phytoconstituents and in vitro anti-oxidant as well as free radical scavenging activity of aqueous root extract of Withania somnifera, commonly known as aswagandha. Phytochemical constituents were measured both by qualitatively and quantitatively using spectrophotometric assays. Total antioxidant capacity by phosphomolybdenum reduction, ferric reducing power, cupric ion reducing power and ferrous chelation assays were measured for determination of anti-oxidant activity. In vitro DPPH radical, superoxide radical, hydrogen peroxide, hydroxyl radical and nitric oxide scavenging activities were also estimated using spectrophotometry. Our study showed that the root extract of W. somnifera contains active phytoconstituents like polyphenols, tannins and alkaloids, terpenoids, saponin and reducing sugars. Furthermore, it showed cupric as well as ferric ion reducing capability and selective scavenging ability towards free radicals such as superoxide anion, nitric oxide and hydrogen peroxide. This study elucidated that root extract of W. somnifera possesses concentration dependent superoxide, nitric oxide and hydrogen peroxide scavenging activity. These results suggested the potentials of W. somnifera as a preventive medicine against free radical associated oxidative assault.
6 , 1 , 2016
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